Engineering > Study Notes > University of California, San Diego - BENG 168beng 168 lecture 7 notes (All)

University of California, San Diego - BENG 168beng 168 lecture 7 notes

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Inducible gene regulation: o can use chemicals/enzymes to destroy (knock out) inhibitors to increase gene expression/product formation  codon optimization o different species have different lev ... els of tRNAs o altering genetic structure/tRNA level can lead to altered expression/production levels  recombinase o cre-loxP - 2 strands can recombine o can use to cut genes out of a sequence or insert genes into a sequence o system is reversible in theory, but in reality is largely irreversible  protein secretion: o clean environment compared to cytoplasm o easier purifications o oxidized environment is important for correct protein folding o proinsulin – 10 times more stable in periplasm than in cytosol o can attach signal peptide to polypeptide chain to induce secretion o YebF can be secreted w/p permeation of membranes -> can be used to help with secretion of choice protein w/o lysing the cell (causes release of lipopolysaccharide [Show More]

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