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Engineering > Study Notes > University of California, San Diego - BENG 168beng 168 lecture 8 notes (All)

University of California, San Diego - BENG 168beng 168 lecture 8 notes

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Pichia pastoris as another fungal model o Compared to s. cerevisiae: less hyperglycosylated, less ethanol production at high density o Can undergo mannose modifications + other molecule additions ... o Genome integration  Engineered His4 deficient cells  AOX1 promoter is regulatable by methanol  Insert from plasmid into gene (?)  Can use histidine (with/without) to pick out the properly engineered yeast  Something to note: gene insertion has a VERY low success rate o A Adeninivorans – alpha-amylase gene  Tryptophan used as a selector marker  Can design with either a strong or weak promoter  Purpose of weaker promoter: allow you to have more copies of gene of interest  Purpose of stronger promoter: allow you to use trp to identify successfully transformed yeast [Show More]

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