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MICRO BIOLOGY PRACTICAL LAB REPORT

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Question 1. Liquid media used for culturing microorganisms in the lab is typically sterilized using a small autoclave (essentially, a programmable pressure cooker). I prepare 5 litres of medium, sp ... lit this into five one-litre bottles and put these into the autoclave. The autoclave is programmed for a 20 minute/121oC cycle. My lazy colleague also makes 5 litres of the same medium, but instead puts the whole lot into a single large bottle and autoclaves this using the same programme. A day-or-so after autoclaving, the medium in my colleague’s bottle has gone cloudy indicating that contaminating bacteria have survived the autoclaving and have grown in the medium. In contrast, the medium in all five of my bottles is clear (i.e. no bacterial growth). Why do you think we have different results? [Show More]

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